Top-ranked Williams failed to convert a match point and lost, 6-2, 6-7 (2), 8-6. No. 7-seed Stosur had ended four-time champion Justine Henin's Roland Garros winning streak at 24 matches in the fourth round.
Paris -- Top-ranked Serena Williams lost in the quarterfinals of the French Open, failing to convert a match point and losing to Australian Samantha Stosur, 6-2, 6-7 (2), 8-6.
The upset Wednesday was the second in a row for the No. 7-seeded Stosur. She ended four-time champion Justine Henin's Roland Garros winning streak at 24 matches in the fourth round.
Against Williams, Stosur used her forceful forehand to build a lead, winning 17 consecutive points during one stretch. Williams rallied and needed only one point for a win in the 10th game of the final set, but her forehand sailed an inch long.
Stosur hit consecutive cross-court winners to break for a 7-6 lead, then served out the victory, hitting service winners on the final three points.